基本信息：

熊二虎，“潇湘学者”特聘教授，博士生导师，湖南大学/美国德克萨斯大学奥斯汀分校联合培养博士(导师：陈金华/Andrew D. Ellington教授)，湖南省杰出青年基金项目(青年A类)获得者 (2025年)，湖南省青年科技人才-荷尖人才 (2022年)，湖南省化学化工学会第十九届青年化学化工奖 (2023年)，湖南省湘江新区青年科技工作者联合会理事 (2024年)， J. Anal. Test.和Interdiscip. Med.期刊青年编委 (2024年)。目前主持国家自然科学基金面上和青年项目各1项，省部级项目多项，授权中国和美国发明专利各1件，申请中国发明专利7件。近年来，共发表学术论文40余篇，其中以第一/通讯作者身份在Nat. Commun., J. Am. Chem. Soc., Angew. Chem. Int. Ed., JACS Au, Natl. Sci. Rev., CCS Chem., Sci. China Chem., ACS Nano, Anal. Chem.等国际权威期刊上发表论文32篇，5篇ESI高被引论文，2篇热点论文。研究成果发表后，受到国内外相关领域著名专家和学者的广泛关注，并在Chem. Rev., Chem. Soc. Rev., Nat. Biomed. Eng.等国际权威期刊上给予大篇幅引用和肯定性评价。

邮箱：xiongerhu2008@163.com

每年招收硕士研究生2~3名，博士研究生1名；欢迎勤奋好学、乐观上进、且有科研梦想的同学加入课题组。

研究方向:

核酸分子体外诊断技术、CRISPR基因编辑技术、纳米生物探针构建等领域。

主持和参与的科研项目： 

国家自然科学基金面上项目，22274046，基于活性可控的CRISPR/Cas系统的一管式可视化即时分子诊断研究，2023.01-2026.12，主持

国家自然科学基金青年项目，21904042，双足DNA步行器诱导的双信号比率型电化学传感器构建及其在miRNA液体活检中的应用，2020.01-2022.12，主持

湖南省杰出青年基金项目（青年A类），2025JJ20018，2025.01-2027.12，主持

湖南省青年科技人才项目（荷尖人才），2022RC1114，2022.09-2025.09，主持

湖南师范大学“潇湘学者”特聘教授启动基金，主持

广东省自然科学基金面上项目，2021A1515010262，利用CRISPR-Cas系统可视化即时检测新型冠状病毒的应用研究，2021.01-2023.12，主持

化学生物传感与计量学国家重点实验室开放课题（湖南大学），CRISPR/Cas基因编辑工具的电化学传感平台构建及其对miRNA的检测，2019004，2020.05-2022.04，主持

国家自然科学基金重大科研仪器研制项目，21727810，高频无极石英晶体阵列生物传感分析仪，2018.01-2022.12，参与

国家自然科学基金重大研究计划培育项目, 91959128，基于CRISPR/Cas识别原理的纸芯片研究及其在肿瘤基因突变、甲基化可视化检测的应用，2020.01-2022.12，参与

代表性研究论文：

1. J. L. Du, H. L. Xu, X. Y. Zhu, K. Y. Long, J. Q. Lang, L. Jiang, E. H. Xiong*, J. W. Liu, R. H. Yang. Robust Peptide-Functionalized Gold Nanoparticles via Ethynyl Bonding for High-Fidelity Bioanalytical Applications. Angew. Chem. Int. Ed. 2025, 64, e202424351.

2. J. L. Ai, J. H. Deng, J. J. Hu, X. X. Pu*, T. Y. Yuan, Y. L. Teng, H. Li, B. J. Chen, J. L. Du, L. Jiang*, X. Y. Chen, E. H. Xiong*, R. H. Yang. PAM-Independent CRISPR-Cas12a System for Specific Assays of Single Nucleotide Variants. JACS Au 2025, 5, 1392–1401.

3. J. Y. Zeng, P. F. Liu, J. L. Du, S. Li, E. H. Xiong*, R. H. Yang. Split crRNA-Motivated Amplification-Free RNA Testing with CRISPR-Cas12a. Sci. China Chem. 2025, 68, 789–801.

4. P. F. Liu, J. Y. Zeng, C. C. Jiang, J. L. Du, L. Jiang*, S. Li, F. X. Zeng*, E. H. Xiong*. Poly(vinylpyrrolidone)-Enhanced CRISPR-Cas System for Robust Nucleic Acid Diagnostics. Anal. Chem. 2024, 96, 15797–15807.

5. P. F. Liu, Y. T. Lin, X. H. Zhuo, J. Y. Zeng, B. L. Chen, Z. Zou, G. H. Liu, E. H. Xiong*, R. H. Yang*. Universal crRNA Acylation Strategy for Robust Photo-Initiated One-Pot CRISPR-Cas12a Nucleic Acid Diagnostics. Angew. Chem. Int. Ed. 2024, 63, e202401486.
   

6. E. H. Xiong, P. F. Liu, R. J. Deng, K. X. Zhang, R. H. Yang*, J. H. Li*. Recent Advances in Enzyme-Free and Enzyme-Mediated Single-Nucleotide Variation Assay in Vitro. Natl. Sci. Rev. 2024, 11, nwae118.

7. Z. Zou*, S. L. Pan, Q. Xue, T. Chen, Z. Y. Huang, B. Qing, P. F. Liu, C. H. Zhao, Y. L. Sun, E. H. Xiong*, R. H. Yang. Artificial Nucleic Acid Tractor-Directed Simultaneous Depletion of Oncogenic Membrane Proteins without Hijacking Proteolysis-Specific Actuator. CCS Chem. 2024, 6, 439–449.

8. L. Jiang, J. L. Du, H. L. Xu, X. H. Zhuo, J. L. Ai, J. Y. Zeng, R. H. Yang, E. H. Xiong*. Ultrasensitive CRISPR/Cas13a-Mediated Photoelectrochemical Biosensors for Specific and Direct Assay of miRNA-21. Anal. Chem. 2023, 95, 1193–1200.

9. M. Q. Huang, E. H. Xiong*, Y. Wang, M. L. Hu, H. H. Yue, T. Tian, D. B. Zhu, H. Liu, X. M. Zhou*. Fast Microwave Heating-Based One-Step Synthesis of DNA and RNA Modified Gold Nanoparticles. Nat. Commun. 2022, 13, 968.

10. E. H. Xiong, L. Jiang, T. Tian, M. L. Hu, H. H. Yue, M. Q. Huang, W. Lin, Y. Z. Jiang, D. B. Zhu, X. M. Zhou*. Simultaneous Dual-Gene Diagnosis of SARS-CoV-2 Based on CRISPR/Cas9-Mediated Lateral Flow Assay. Angew. Chem. Int. Ed. 2021, 60, 5307–5315. (ESI Highly cited paper, Hot paper)

11. M. L. Hu, C. Q. Yuan, T. Tian, X. S. Wang, J. Sun, E. H. Xiong*, X. M. Zhou*. Single-Step, Salt-Aging-Free, and Thiol-Free Freezing Construction of AuNP-Based Bioprobes for Advancing CRISPR-Based Diagnostics. J. Am. Chem. Soc. 2020, 142, 7506–7513.

12. X. S. Wang, E. H. Xiong*, T. Tian, M. Cheng, W. Lin, H. Wang, G. H. Zhang, J. Sun*, X. M. Zhou* Clustered Regularly Interspaced Short Palindromic Repeats/Cas9-Mediated Lateral Flow Nucleic Acid Assay. ACS Nano 2020, 14, 2497–2508. (ESI Highly cited paper)

13. E. H. Xiong, D. B. Yao, A. D. Ellington*, S. Bhadra*. Minimizing Leakage in Stacked Strand Exchange Amplification Circuits. ACS Synth. Biol. 2021, 10, 1277–1283.

14. Y. J. Bao, Y. Z. Jiang, E. H. Xiong*, T. Tian, Z. Z. Zhang, J. Lv, Y. Li*, X. M. Zhou*. CUT-LAMP: Contamination-Free Loop-Mediated Isothermal Amplification Based on the CRISPR/Cas9 Cleavage. ACS Sens. 2020, 5, 1082–1091.

15. E. H. Xiong, D. S. Zhen, L. Jiang*, X. M. Zhou*. Binding-Induced 3D-Bipedal DNA Walker for Cascade Signal Amplification Detection of Thrombin Combined with Catalytic Hairpin Assembly Strategy. Anal. Chem. 2019, 91, 15317–15324.

16. E. H. Xiong, D. S. Zhen, L. Jiang*. Homogeneous Enzyme-Free and Entropy-Driven Isothermal Fluorescent Assay for Nucleic Acids Based on a Dual-Signal Output Amplification Strategy. Chem. Commun. 2018, 54, 12594–12597.

17. E. H. Xiong, Z. Z. Li, X. H. Zhang*, J. W. Zhou, X. X. Yan, Y. Q. Liu, J. H. Chen*. Triple-Helix Molecular Switch Electrochemical Ratiometric Biosensor for Ultrasensitive Detection of Nucleic Acids. Anal. Chem. 2017, 89, 8830–8835.

18. E. H. Xiong, X. H. Zhang, Y. Q. Liu, J. W. Zhou, P. Yu, X. Y. Li, J. H. Chen*. Ultrasensitive Electrochemical Detection of Nucleic Acids Based on the Dual-Signaling Electrochemical Ratiometric Method and Exonuclease III-Assisted Target Recycling Amplification Strategy. Anal. Chem. 2015, 87, 7291–7296.

19. E. H. Xiong, X. X. Yan, X. H. Zhang*, Y. Q. Liu, J. W. Zhou, J. H. Chen*. Exonuclease III–Assisted Cascade Signal Amplification Strategy for Label-Free and Ultrasensitive Electrochemical Detection of Nucleic Acids. Biosens. Bioelectron. 2017, 87, 732–736.

20. E. H. Xiong, L. Wu, J. W. Zhou, P. Yu, X. H. Zhang*, J. H. Chen*. A Ratiometric Electrochemical Biosensor for Sensitive Detection of Hg2+Based on Thymine-Hg2+-Thymine Structure. Anal. Chim. Acta 2015, 853, 242–248.

21. D. B. Yao, S. Bhadra, E. H. Xiong, H. J. Liang, A. D. Ellington*, C. Jung*. Dynamic Programming of a DNA Walker Controlled by Protons. ACS Nano 2020, 14, 4007–4013.

22. H. H. Yue, M. Q. Huang, T. Tian, E. H. Xiong, X. M. Zhou*. Advances in Clustered, Regularly Interspaced Short Palindromic Repeats (CRISPR)-Based Diagnostic Assays Assisted by Micro/Nanotechnologies. ACS Nano 2021, 15, 7848–7859.